PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASES-C OF BULL AND RABBIT SPERMATOZOA

被引:18
作者
HINKOVSKAGALCHEV, V [1 ]
SRIVASTAVA, PN [1 ]
机构
[1] UNIV GEORGIA, DEPT BIOCHEM, LIFE SCI BLDG, ATHENS, GA 30602 USA
关键词
SPERM; (BULL; RABBIT); PLASMA MEMBRANES; ACROSOMAL MEMBRANES; PHOSPHATIDYLINOSITOL SPECIFIC PHOSPHOLIPASE-C; PHOSPHATIDYL CHOLINE SPECIFIC PHOSPHOLIPASE-C;
D O I
10.1002/mrd.1080330308
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Acrosomal reaction is an essential prerequisite to fertilization. The changes in lipid composition of sperm membranes cause fusion of the plasma and outer acrosomal membranes that results in the exocytosis of acrosomal contents. We report that both bull and rabbit spermatozoa contain a phosphatidylcholine-specific phospholipase C (PC-PLC) that hydrolyzes L-alpha-dipalmitoyl-(choline-methyl-C-14-153.0 Ci/mmol and a phosphatidyl-inositol-specific phospholipase C (PI-PLC) that hydrolyzes L-alpha-(Myo-Inositol-2-H-3 (N)-5.2 Ci mmol. PI-PLC from bull sperm acrosome has been purified 568 x fold with a specific activity 6.25 +/- 0.6 nmol/min/mg protein, k(m) 0.004 mM, and V(max) 12 nmol/min/mg protein. Both enzymes had optimum at pH 7.5. The activity of PC-PLC remained unaffected by varying concentrations of Ca2+ whereas PI activity was significantly increased. The bulk of PI-PLC was found to be associated with inner acrosomal membrane of bull and rabbit sperm, while PC-PLC was found in the outer acrosomal membranes in the bull sperm and the plasma membrane of the rabbit sperm. Both enzymes are compartmentalized in sperm cell.
引用
收藏
页码:281 / 286
页数:6
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