BIOPOLYMERS FROM A-LATUS .3. CULTURE CONDITIONS FOR PRODUCTION AND PURIFICATION OF BIOABSORBENT FROM ALCALIGENES LATUS B-16

被引：8

作者：

NOHATA, Y

KURANE, R

机构：

[1] MINIST INT TRADE & IND,AGCY IND SCI & TECHNOL,NATL INST BIOSCI & HUMAN TECHNOL,TSUKUBA,IBARAKI 305,JAPAN

[2] HAKUTO CO LTD,YOKKAICHI LAB,YOKKAICHI,MIE,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1994年 / 77卷 / 04期

关键词：

Algae - Bacteria - Carbon - Electrophoresis - Gel permeation chromatography - Glucose - Plant cell culture - Polysaccharides - Purification - Substrates - Sugar (sucrose) - Water absorption;

D O I：

10.1016/0922-338X(94)90010-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glucose and sucrose were favorable carbon sources for the production of bioabsorbent from Alcaligenes latus B-16. Yeast extract at a concentration of 0.5% was also an effective medium for growth. Biopolymers from A. latus B-16 were purified, and the biopolymers were separated into two types of biopolymers: a high-molecular weight component (acid-biopolymer) and a low-molecular weight component (neutral-biopolymer). These two types of biopolymers were shown to be homogeneous on electrophoresis and GPC (Gel Permeation Chromatography on HPLC). The high-molecular weight component was bioabsorbent, and was able to absorb water at more than 1,000 times its own weight. The total yield of acid- and neutral-biopolymers from A. latus B-16 was 20-25 g (dry weight) per liter of culture broth, and they were produced at a ratio of 20:1 (acid:neutral).

引用

页码：390 / 393

页数：4

共 7 条

[1]

Chaplin M F, 1986, CARBOHYDRATE ANAL PR, P129

[2]

ISHIZU A, 1984, DEV FUNCTIONAL CELLU, P50

[3] BIOPOLYMER FROM ALCALIGENES-LATUS .1. MICROBIAL FLOCCULATION OF WASTE LIQUIDS AND OIL EMULSION BY A BIOFLOCCULANT FROM ALCALIGENES-LATUS [J].