A SERINE (THREONINE) PROTEIN-KINASE CONFERS FUNGICIDE RESISTANCE IN THE PHYTOPATHOGENIC FUNGUS USTILAGO-MAYDIS

被引：45

作者：

ORTH, AB

RZHETSKAYA, M

PELL, EJ

TIEN, M

机构：

[1] PENN STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,UNIVERSITY PK,PA 16802

[2] PENN STATE UNIV,DEPT PLANT PATHOL,UNIVERSITY PK,PA 16802

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 06期

关键词：

D O I：

10.1128/AEM.61.6.2341-2345.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance-conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.

引用

页码：2341 / 2345

页数：5

共 22 条

[1] COMPLETE NUCLEOTIDE-SEQUENCE OF A GENE CONFERRING POLYMYXIN-B RESISTANCE ON YEAST - SIMILARITY OF THE PREDICTED POLYPEPTIDE TO PROTEIN-KINASES [J].