SENSITIVE MICRO-ASSAY FOR THE MURINE ALTERNATIVE COMPLEMENT PATHWAY

被引：17

作者：

JOINER, KA

SHAHON, R

GELFAND, JA

机构：

[1] TUFTS UNIV,NEW ENGLAND MED CTR,DEPT MED,DIV EXPTL MED,BOSTON,MA 02111

[2] TUFTS UNIV,NEW ENGLAND MED CTR,DEPT MED,DIV INFECT DIS,BOSTON,MA 02111

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 31卷 / 3-4期

关键词：

D O I：

10.1016/0022-1759(79)90141-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microhemolytic assay for measurement of murine alternative complement pathway activity is described. The assay uses 51Cr release from neuraminidase-treated rabbit erythrocytes incubated with Mg2+ EGTA-chelated murine serum. Neuraminidase pretreatment of rabbit erythrocytes increases the sensitivity of the assay 8-10-fold, enabling the use of small volumes of individual mouse sera. The assay affords a simple, sensitive and reproducible method for measuring murine alternative complement pathway activity. Significant differences were found between strains when alternative complement pathway activity of serum from various inbred murine lines was measured. © 1979.

引用

页码：283 / 290

页数：8

共 25 条

[1] EFFECT OF SIALIDASE ON VIABILITY OF ERYTHROCYTES IN CIRCULATION [J].