SYNTHESIS OF ENKEPHALINS BY GUINEA-PIG STRIATUM INVITRO

被引：14

作者：

MCKNIGHT, AT

HUGHES, J

KOSTERLITZ, HW

机构：

来源：

PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES | 1979年 / 205卷 / 1159期

关键词：

D O I：

10.1098/rspb.1979.0060

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method for obtaining incorporation of labelled amino acids into enkephalins of guinea-pig striatum in vitro and isolating the labelled enkephalins is described. The incorporation of [3H]tyrosine increases with time after a delay of 2 h. Cycloheximide added during this time, but not at later stages, inhibits incorporation. The results indicate that the enkephalins are produced locally, probably by ribosomal synthesis. The lag period may be due to the time required for the synthesis of precursors and their conversion to the enkephalins.

引用

收藏

页码：199 / 207

页数：9

相关论文

共 13 条

[1]

CLOUET DH, 1976, OPIATES ENDOGENOUS O, P71

[2] IMMUNOHISTOCHEMICAL STUDIES USING ANTIBODIES TO LEUCINE-ENCEPHALIN - INITIAL OBSERVATIONS ON NERVOUS-SYSTEM OF RAT [J].

ELDE, R ;

HOKFELT, T ;

JOHANSSON, O ;

TERENIUS, L .

NEUROSCIENCE, 1976, 1 (04) :349-&

[3] REGIONAL STUDIES OF CATECHOLAMINES IN RAT BRAIN .I. DISPOSITION OF [3H]NOREPINEPHRINE [3H]DOPAMINE AND [3H]DOPA IN VARIOUS REGIONS OF BRAIN [J].

GLOWINSKI, J ;

IVERSEN, LL .

JOURNAL OF NEUROCHEMISTRY, 1966, 13 (08) :655-+

[4] DISTRIBUTION OF METHIONINE-ENKEPHALIN AND LEUCINE-ENKEPHALIN IN BRAIN AND PERIPHERAL TISSUES [J].

HUGHES, J ;

KOSTERLITZ, HW ;

SMITH, TW .

BRITISH JOURNAL OF PHARMACOLOGY, 1977, 61 (04) :639-647

[5] IDENTIFICATION OF 2 RELATED PENTAPEPTIDES FROM BRAIN WITH POTENT OPIATE AGONIST ACTIVITY [J].

HUGHES, J ;

SMITH, TW ;

KOSTERLITZ, HW ;

FOTHERGILL, LA ;

MORGAN, BA ;

MORRIS, HR .

NATURE, 1975, 258 (5536) :577-579

[6]

HUGHES J, 1978, BRIT J PHARMACOL, V63, pP396

[7] EVIDENCE FOR PRECURSOR IN VASOPRESSIN BIOSYNTHESIS [J].

SACHS, H ;

TAKABATAKE, Y .

ENDOCRINOLOGY, 1964, 75 (06) :943-&

[8] OPIOID PEPTIDE ENKEPHALIN - IMMUNOHISTOCHEMICAL MAPPING IN RAT CENTRAL NERVOUS-SYSTEM [J].

SIMANTOV, R ;

KUHAR, MJ ;

UHL, GR ;

SNYDER, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (05) :2167-2171

[9] ISOLATION AND STRUCTURE IDENTIFICATION OF A MORPHINE-LIKE PEPTIDE ENKEPHALIN IN BOVINE BRAIN [J].

SIMANTOV, R ;

SNYDER, SH .

LIFE SCIENCES, 1976, 18 (08) :781-788

[10] INCORPORATION OF LABELED AMINO-ACIDS INTO ENKEPHALINS [J].

SOSA, RP ;

MCKNIGHT, AT ;

HUGHES, J ;

KOSTERLITZ, HW .

FEBS LETTERS, 1977, 84 (01) :195-198