PHOSPHOLIPID ALKANETHIOL BILAYERS FOR CELL-SURFACE RECEPTOR STUDIES BY SURFACE-PLASMON RESONANCE

被引:164
作者
PLANT, AL
BRIGHAMBURKE, M
PETRELLA, EC
OSHANNESSY, DJ
机构
[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT PROT BIOCHEM,KING OF PRUSSIA,PA 19406
[2] JOHNS HOPKINS UNIV,SCH MED,DEPT ANAT & CELL BIOL,BALTIMORE,MD 21205
关键词
D O I
10.1006/abio.1995.1234
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Supported hybrid bilayer membranes (HBM) composed of a monolayer of phospholipid and a monolayer of alkanethiol associated with a thin gold film on glass are useful as model lipid bilayer membranes for studying membrane receptor-ligand and cell-cell binding events by surface plasmon resonance (SPR). Measurements of specific binding of proteins and lipid vesicles to well-defined HBMs have been performed under conditions of continuous flow using a commercial SPR instrument (BIAcore). HBMs are shown to be stable in how and to block nonspecific adsorption of proteins to the alkanethiol/gold surface. The use of such supported lipid bilayers in flow provides a means of conducting equilibrium and kinetic studies of models of ligand-cell and cell-cell interactions with receptors or ligands in a membrane environment. Compared to the extended dextran polymer layer that is currently used for surface modification of BIAcore ''sensor chips,'' the described HBMs provide a well-defined surface that will permit less ambiguous modeling of these important biological interactions. (C) 1995 Academic Press, Inc.
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页码:342 / 348
页数:7
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