EPITOPE TAG VECTORS FOR EUKARYOTIC PROTEIN-PRODUCTION

被引：72

作者：

SELLS, MA ^{[1
]}

CHERNOFF, J ^{[1
]}

机构：

[1] FOX CHASE CANC CTR, PHILADELPHIA, PA 19111 USA

来源：

GENE | 1995年 / 152卷 / 02期

关键词：

TRANSFECTION; PLASMID; COS CELLS; IMMUNOBLOT; IMMUNOPRECIPITATION;

D O I：

10.1016/0378-1119(94)00685-L

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report on the construction and use of two eukaryotic expression vectors which add well-characterized epitope tags to the N termini of proteins. The utility of these Vectors is demonstrated for detecting the expression of a variety of proteins. As the addition of these epitope tags can in some cases obviate the need to generate specific antisera to each individual protein, these vectors provide a facile means both to monitor protein expression and to purify such expressed proteins.

引用

页码：187 / 189

页数：3

共 7 条

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