DNA-FINGERPRINTING REVEALS SIGNIFICANT AMOUNTS OF GENETIC-VARIATION IN A WILD RASPBERRY RUBUS-IDAEUS POPULATION

被引:39
作者
ANTONIUS, K
NYBOM, H
机构
[1] SWEDISH UNIV AGR SCI,DEPT HORT PLANT BREEDING,S-29194 KRISTIANSTAD,SWEDEN
[2] UNIV HELSINKI,DEPT PLANT BIOL PLANT BREEDING,SF-00014 HELSINKI,FINLAND
关键词
APOMIXIS; BLACKBERRY; BREEDING SYSTEM; DNA FINGERPRINTING; RASPBERRY; VEGETATIVE PROPAGATION;
D O I
10.1111/j.1365-294X.1994.tb00119.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Establishing the genotypic distribution in natural plant populations is an important part of ecological studies concerning plant growth, reproduction and turn-over. Restriction enzyme-digested DNA samples, isolated from 24 plants of a natural Rubus idaeus population, were analysed with DNA fingerprinting using the M13 repeat sequence as well as a synthetic (AC)/(TG) polydinucleotide as hybridization probes. All the examined samples exhibit unique DNA fingerprint patterns, suggesting that vegetative reproduction may be considerably more restricted in wild R. idaeus populations than previously assumed. By comparison, all samples of the apomictic blackberry species Rubus nessensis, collected on the same location, were completely identical.
引用
收藏
页码:177 / 180
页数:4
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