QUANTITATIVE THIN-LAYER CHROMATOGRAPHY - THIN-FILM FLUORESCENCE SCANNING ANALYSIS OF ADRIAMYCIN AND METABOLITES IN TISSUE

被引：21

作者：

CHAN, KK

WONG, CD

机构：

[1] School of Pharmacy, University of Southern California, Los Angeles

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 172卷 / APR期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0021-9673(00)90971-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid, non-hydrolytic thin-film fluorescence scanning method is described for the quantitation of adriamycin and metabolites in tissues. Adriamycin, with daunomycin added as the internal standard, was extracted from tissue homogenate which contained 500 μg of oxalic acid, with ice-cold 0.5 N hydrochloric acid-85% isopropanol, separated by thin-layer chromatography, and quantiated in situ via a fluorescence scanning technique. This method has a sensitivity to 0.05 μg per gram of tissue. © 1979.

引用

页码：343 / 349

页数：7

共 8 条

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[2] DAUNORUBICIN AND ADRIAMYCIN METABOLISM IN GOLDEN SYRIAN-HAMSTER
BACHUR, NR
EGORIN, MJ
HILDEBRAND, RC
[J]. BIOCHEMICAL MEDICINE, 1973, 8 (03): : 352 - 361
[3] CHAN KK, 1973, RES COMMUN CHEM PATH, V6, P447
[4] QUANTITATIVE THIN-LAYER CHROMATOGRAPHIC ASSAY FOR BETA-NEOAGAROTETRAOSE HYDROLASE OF PSEUDOMONAS-ATLANTICA
GROLEAU, D
YAPHE, W
[J]. JOURNAL OF CHROMATOGRAPHY, 1977, 137 (01): : 234 - 239
[5] THIN-LAYER CHROMATOGRAPHIC ANALYSIS OF PHENYTOIN AND ITS HYDROXY METABOLITES
RAO, GS
MCLENNON, DA
[J]. JOURNAL OF CHROMATOGRAPHY, 1977, 137 (01): : 231 - 233
[6] NEW FLUORIMETRIC PROCEDURE FOR DETECTION AND QUANTITATION OF ORGANIC COMPOUNDS IN THIN-LAYER CHROMATOGRAPHY
SEGURA, R
GOTTO, AM
[J]. JOURNAL OF CHROMATOGRAPHY, 1974, 99 (NOV6): : 643 - 657
[7] TOUCHSTONE JC, 1973, QUANTITATIVE THIN LA
[8] WATSON E, 1976, CANCER TREAT REP, V60, P1611

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