USE OF MONOCLONAL-ANTIBODIES TO RECOGNIZE OSTEOCLASTS IN ROUTINELY PROCESSED BONE-BIOPSY SPECIMENS

被引：21

作者：

ATHANASOU, NA ^{[1
]}

PUDDLE, B ^{[1
]}

QUINN, J ^{[1
]}

WOODS, CG ^{[1
]}

机构：

[1] NUFFIELD ORTHOPAED CTR,OXFORD OX3 7LD,ENGLAND

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 44卷 / 08期

关键词：

D O I：

10.1136/jcp.44.8.664

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

In decalcified (5% nitric acid) and undecalcified (glycol-methacrylate or resin embedded) routinely processed bone specimens osteoclasts against resorbing surfaces were identified with monoclonal antibodies directed against leucocyte common antigen (LCA) (PD7/26, 2B11), CD68 (KP1), and gpIIIa (Y2/51) but not against HLA-DR (CR3/43 and Ta11B5). Mononuclear cells on resorbing surfaces and occasional mononuclear cells against or near resting surfaces showed a similar pattern of reactivity. This study shows that immunohistochemistry is a sensitive and useful technique for identifying osteoclasts in routinely processed bone specimens. It also suggests a role for mononuclear cells (possibly pre-osteoclasts) in bone resorption.

引用

页码：664 / 666

页数：3

共 17 条

[1] IMMUNOPHENOTYPIC DIFFERENCES BETWEEN OSTEOCLASTS AND MACROPHAGE POLYKARYONS - IMMUNOHISTOLOGICAL DISTINCTION AND IMPLICATIONS FOR OSTEOCLAST ONTOGENY AND FUNCTION [J].