A PURIFICATION STRATEGY FOR INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE FROM RAT-LIVER BASED UPON HEPARIN INTERACTION CHROMATOGRAPHY

被引：12

作者：

CONIGRAVE, A

PATWARDHAN, A

BROOMHEAD, L

ROUFOGALIS, B

机构：

[1] UNIV SYDNEY,DEPT BIOCHEM,SYDNEY,NSW 2006,AUSTRALIA

[2] UNIV SYDNEY,DEPT PHYSIOL,SYDNEY,NSW 2006,AUSTRALIA

来源：

CELLULAR SIGNALLING | 1992年 / 4卷 / 03期

关键词：

INS (1,3,4,5)P(4); INS (1,4,5)P(3) 3-KINASE; CALMODULIN; HEPARIN; ENZYME PURIFICATION; RAT LIVER; PYROPHOSPHATE;

D O I：

10.1016/0898-6568(92)90070-O

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rat liver inositol 1,4,5-trisphosphate [Ins (1,4,5)P3] 3-kinase was purified in high yield by a three-step procedure reliant upon chromatography on heparin and calmodulin agarose. Purified enzyme was' stable in the presence of the detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS) (0.1-0.5%) and the sulphhydryl reducing reagent dithiothreitol (DTT). The purified enzyme was activated 2-3-fold by Ca2+ (1-mu-M) in the presence of calmodulin. Pyrophosphate and heparin were identified as inhibitors of the enzyme.

引用

页码：303 / &

共 16 条

[1] GTP-GAMMA-S-STIMULATED HYDROLYSIS OF PHOSPHATIDYINOSITOL-4,5-BISPHOSPHATE BY SOLUBLE PHOSPHOLIPASE-C FROM HUMAN-PLATELETS REQUIRES SOLUBLE GTP-BINDING PROTEIN
BALDASSARE, JJ
KNIPP, MA
HENDERSON, PA
FISHER, GJ
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 154 (01) : 351 - 357
[2] RAPID FORMATION OF INOSITOL 1,3,4,5-TETRAKISPHOSPHATE FOLLOWING MUSCARINIC RECEPTOR STIMULATION OF RAT CEREBRAL CORTICAL SLICES
BATTY, IR
NAHORSKI, SR
IRVINE, RF
[J]. BIOCHEMICAL JOURNAL, 1985, 232 (01) : 211 - 215
[3] STIMULATION OF HEPATIC INOSITOL 1,4,5-TRISPHOSPHATE KINASE-ACTIVITY BY CA-2+-DEPENDENT AND CA-2+-INDEPENDENT MECHANISMS
BIDEN, TJ
ALTIN, JG
KARJALAINEN, A
BYGRAVE, FL
[J]. BIOCHEMICAL JOURNAL, 1988, 256 (03) : 697 - 701
[4] CONIGRAVE D, 1989, P AUST BIOCHEM SOC, V21, pP80
[5] HEPARIN INHIBITS THE INOSITOL 1,4,5-TRISPHOSPHATE-INDUCED CA-2+ RELEASE FROM RAT-LIVER MICROSOMES
CULLEN, PJ
COMERFORD, JG
DAWSON, AP
[J]. FEBS LETTERS, 1988, 228 (01) : 57 - 59
[6] GUILLEMETTE G, 1989, MOL PHARMACOL, V35, P339
[7] HANSEN CA, 1986, J BIOL CHEM, V261, P8100
[8] HEPARIN INHIBITS INOSITOL TRISPHOSPHATE-INDUCED CALCIUM RELEASE FROM PERMEABILIZED RAT-LIVER CELLS
HILL, TD
BERGGREN, PO
BOYNTON, AL
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 149 (03) : 897 - 901
[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[10] LEE SY, 1990, J BIOL CHEM, V265, P9434

← 1 2 →