PRODUCTION AND CHARACTERIZATION OF AN IMINOIMIDAZOLIDINE SPECIFIC MONOCLONAL-ANTIBODY USING PARA-AMINOCLONIDINE AS ANTIGEN

被引：14

作者：

DONTENWILL, M

MOLINES, A

BRICCA, G

STUTZMAN, J

KEMPF, J

BELCOURT, A

BOUSQUET, P

机构：

[1] Laboratoire de Pharmacologie Cardiovasculaire et Rénale, CNRS URA 589, Faculté de Médecine, 67000 Strasbourg

来源：

LIFE SCIENCES | 1992年 / 50卷 / 24期

关键词：

D O I：

10.1016/0024-3205(92)90546-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Para-aminoclonidine coupled to hemocyanin was used to produce mouse monoclonal antibodies directed against clonidine. The properties of one of these, called mFE7, secreted by a clone of hybrid myeloma, are described. This antibody displayed total crossreactivity with imidazolidines and no crossreactivity at all with catecholamines or other known naturally occuring substances tested. A liquid phase radioimmunoassay permitted the detection of immunoreactivity in human brain extracts. The mFE7 antibody could be useful for immunopurifying the endogenous ligand of Imidazolines Preferring Receptors (IPR) which are catecholamines insensitive.

引用

页码：1859 / 1868

页数：10

共 31 条

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