INDUCTION OF CHROMOSOMAL-ABERRATIONS IN CHINESE-HAMSTER OVARY CELLS BY TRIETHYLLEAD ACETATE

被引:6
作者
BLAKEY, DH
BAYLEY, JM
DOUGLAS, GR
机构
来源
MUTATION RESEARCH | 1992年 / 298卷 / 01期
关键词
CHINESE HAMSTER OVARY CELLS; TRIETHYLLEAD ACETATE; ORGANOLEAD COMPOUNDS; GASOLINE; LEADED; ALKYLLEADS;
D O I
10.1016/0165-1218(92)90022-R
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Organolead compounds enter the environment primarily through the combustion of leaded gasoline and industrial discharge. Lead and lead-containing compounds have been shown to induce a broad spectrum of toxic effects, including hematopoetic, renal, neurologic, and carcinogenic effects. In this study, the mutagenic activity of triethyllead acetate (Et3PbAc) was determined by measuring the induction of chromosomal aberrations in Chinese hamster ovary cells. The results indicate that Et3PbAc is very cytotoxic and a potent clastogen. In preliminary cytotoxicity studies used to determine appropriate test concentrations for chromosomal aberration analysis, the LC50 of Et3PbAc was approximately 10 muM in the absence of metabolic activation, and 80 muM in the presence of metabolic activation. Et3PbAc induced a significant (p < 0.01) increase in chromosomal aberrations at 6 muM in the absence of metabolic activation, and 40 muM in the presence of metabolic activation. The maximal response was greater with metabolic activation than without. However, a much higher dose was required to elicit a significant response in the presence of metabolic activation than in its absence.
引用
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页码:1 / 7
页数:7
相关论文
共 26 条
[1]  
ANAGNOSTOPOULOS A, 1983, LEAD VERSUS HLTH, P107
[2]  
BILLICK IH, 1983, LEAD VERSUS HLTH, P59
[3]  
CHAU YK, 1984, BIOL EFFECTS ORGANOL, P21
[4]  
CREMER J E, 1959, Br J Ind Med, V16, P191
[5]   LEAD IN URBAN STREET DUST [J].
DAY, JP ;
HART, M ;
ROBINSON, MS .
NATURE, 1975, 253 (5490) :343-345
[6]   SOLUBILITY AND POTENTIAL TOXICITY OF LEAD IN URBAN STREET DUST [J].
DAY, JP ;
FERGUSSON, JE ;
CHEE, TM .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1979, 23 (4-5) :497-502
[7]  
FRANKLIN CA, 1987, TRACE SUBSTANCES ENV, V21, P286
[8]   EFFECTS OF HIGH OSMOTIC STRENGTH ON CHROMOSOME-ABERRATIONS, SISTER-CHROMATID EXCHANGES AND DNA STRAND BREAKS, AND THE RELATION TO TOXICITY [J].
GALLOWAY, SM ;
DEASY, DA ;
BEAN, CL ;
KRAYNAK, AR ;
ARMSTRONG, MJ ;
BRADLEY, MO .
MUTATION RESEARCH, 1987, 189 (01) :15-25
[9]   TOXICITY, MUTAGENICITY AND TERATOGENICITY OF LEAD [J].
GERBER, GB ;
LEONARD, A ;
JACQUET, P .
MUTATION RESEARCH, 1980, 76 (02) :115-141
[10]   CURRENT STATUS OF BIOASSAYS IN GENETIC TOXICOLOGY - THE DOMINANT LETHAL ASSAY - A REPORT OF THE US-ENVIRONMENTAL-PROTECTION-AGENCY GENE-TOX PROGRAM [J].
GREEN, S ;
AULETTA, A ;
FABRICANT, J ;
KAPP, R ;
MANANDHAR, M ;
SHEU, CJ ;
SPRINGER, J ;
WHITFIELD, B .
MUTATION RESEARCH, 1985, 154 (01) :49-67