IMMOBILIZED YEAST-CELLS BIOSENSOR FOR TOTAL TOXICITY TESTING

被引:34
作者
CAMPANELLA, L
FAVERO, G
TOMASSETTI, M
机构
[1] Dipartimento di Chimica, Università 'La Sapienza', 00185 Rome, Roma P.le Aldo Moro
关键词
CELL BIOSENSOR; YEAST; TOTAL TOXICITY TESTING;
D O I
10.1016/0048-9697(95)04673-0
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
An immobilised yeast cell biosensor has been developed for the total toxicity testing of a sample that may contain a number of different pollutant species; the biosensor uses an amperometric gas diffusion oxygen as indicator electrode. The method is based on the perturbation of the respiratory activity of a yeast, Saccharomyces cerevisiae, immobilised on an agar gel containing the culture medium (i.e., 'agarised medium'), by the toxic test substance. Glucose is used as substrate while the toxic substances tested consist of several metallic ions, phenol and cationic or anionic surfactants.
引用
收藏
页码:227 / 234
页数:8
相关论文
共 14 条
  • [1] CAMPANELLA L, 1993, NATO ADV SCI INST SE, V252, P489
  • [2] CAMPANELLA L, 1986, INQUINAMENTO, V10, P54
  • [3] CAMPANELLA L, 1992, MET ANAL ACQUE, V12, P23
  • [4] ACUTE TOXICITY SCREENING OF WATER POLLUTANTS USING A BACTERIAL ELECTRODE
    DORWARD, EJ
    BARISAS, BG
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1984, 18 (12) : 967 - 972
  • [5] HUNTER JB, 1978, WATER POLLUT CONTROL, V77, P384
  • [6] KARUBE I, 1984, ION SEL ELECTRODE R, V6, P15
  • [7] KEULERS M, 1993, IDENTIFICATION CONTR, P14
  • [8] MARCHETTI R, 1991, QUAD I RIC ACQUE, V93
  • [9] PRICE DRH, 1978, WATER POLLUT CONTROL, V50, P285
  • [10] WHOLE-CELL BIOSENORS FOR ENVIRONMENTAL MONITORING
    RAWSON, DM
    WILLMER, AJ
    TURNER, APF
    [J]. BIOSENSORS, 1989, 4 (05) : 299 - 311