GAS-CHROMATOGRAPHIC ESTIMATION OF URINARY VANILLYLMANDELIC ACID AFTER TRANSFORMATION TO VANILLIN METHOXIME TRIMETHYLSILYL ETHER

被引：16

作者：

KAHANE, Z

MOWAT, JH

VESTERGA.P

机构：

[1] Research Center, Rocklcmd State Hospital, Orangeburg, N.Y

来源：

CLINICA CHIMICA ACTA | 1969年 / 26卷 / 02期

关键词：

D O I：

10.1016/0009-8981(69)90384-2

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A method is described for urinary vanillylmandelic acid (VMA). After initial extraction into ethyl acetate the VMA is oxidized with sodium periodate to form vanillin. The vanillin is transformed first to an o-methyl oxime by reaction with methoxyamine hydrochloride in pyridine. This is followed by trimethylsilyl ether formation. The o-methyl oxime-trimethylsilyl ether compound can be quantitated at low temperature on a gas chromatograph with good recovery. Specificity of the assay is documented by the use of combined gas chromatography and mass spectrometry. © 1969.

引用

页码：307 / &

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