GENERATION OF VARIANCE, THEORETICAL PLATES, RESOLUTION, AND PEAK CAPACITY IN ELECTROPHORESIS AND SEDIMENTATION

被引:259
作者
GIDDINGS, JC
机构
[1] Department of Chemistry, University of Utah Salt, Lake City, Utah
来源
SEPARATION SCIENCE | 1969年 / 4卷 / 03期
关键词
D O I
10.1080/01496396908052249
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The rate of generation of variance, dσ2/dX, is a fundamental parameter which determines peak or boundary width and, thus, resolution in many differential migration systems. This parameter can be identified with the “plate height” of chromatography. By extending this nomenclature and the underlying concepts to electrophoresis and sedimentation, we arrive at parameters, particularly the “number of theoretical plates,” which allow a comparison of the effectiveness of these diverse methods. Equations are derived for the plate number as well as for resolution and peak capacity. Numerical comparisons are shown. Optimization is discussed with reference to maximum resolution, peak capacity, and separation speed. © 1969, Taylor & Francis Group, LLC. All rights reserved.
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页码:181 / &
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