EXTRACTION OF PROTEINS AND PEPTIDES FROM COOMASSIE BLUE-STAINED SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS

被引：25

作者：

GIBSON, DR

GRACY, RW

机构：

[1] TEXAS COLL OSTEOPATH MED, FT WORTH, TX 76107 USA

[2] N TEXAS STATE UNIV, HLTH SCI CTR, DEPT BIOCHEM, FT WORTH, TX 76107 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90592-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for extracting proteins and peptides from stained sodecyl sulfate-polyacrylamide gels. Coomassie blue and sodium dodecyl sulfate present in stained gel sections are removed to allow subsequent analysis of the peptides (e.g., amino acid analysis or tryptic digestion and fingerprinting). The method is simple, requires no radioisotopes or special equipment, and can be carried out with a minimum of handling of the sample. The process can be used for samples at the nanomole level with recoveries of 90%. © 1979.

引用

页码：352 / 354

页数：3