A 150 KILODALTON CELL-SURFACE PROTEIN IS INDUCED BY SALT IN THE HALOTOLERANT GREEN-ALGA DUNALIELLA-SALINA

被引:49
作者
SADKA, A
HIMMELHOCH, S
ZAMIR, A
机构
[1] WEIZMANN INST SCI,DEPT BIOCHEM,IL-76100 REHOVOT,ISRAEL
[2] WEIZMANN INST SCI,BIOL SERV,IL-76100 REHOVOT,ISRAEL
关键词
D O I
10.1104/pp.95.3.822
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Dunaliella salina is an extremely halotolerant, unicellular, green alga lacking a rigid cell wall. Osmotic adaptation to high salinities is based on the accumulation of glycerol. To uncover other functions responsible for halotolerance, protein profiles of algae continuously grown in different salinities were compared. A 150 kilodalton protein (p 150) increased in amount with salt concentration. Furthermore, when the cells were subjected to drastic hyperosmotic shocks, p 150 started to rise long after completion of the osmotic response but coincident with reinitiation of cell proliferation. Cells with an initially higher level of p 150 resumed growth faster than cells with a lower level of the protein. Addition of cycloheximide early after hyperosmotic shock prevented the rise in p 150, indicating this rise was due to de novo synthesis of the protein. These observations suggest that p 150 is a salt-induced protein required for proliferation of the cells in saline media. p 150 was purified to homogeneity and found to be a detergent-soluble glycoprotein. Polyclonal antibodies against p 150 recognized a single protein component in D. salina crude extracts. A high M(r) cross-reacting protein was also observed in another Dunaliella strain, D. bardawil. Immunoelectron microscopy localized p 150 to the cell surface.
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页码:822 / 831
页数:10
相关论文
共 32 条
[1]   THE OSMOTIC COMPONENTS OF HALOTOLERANT ALGAE [J].
AVRON, M .
TRENDS IN BIOCHEMICAL SCIENCES, 1986, 11 (01) :5-6
[2]   ENZYME-BASED DETECTION OF GLYCOPROTEINS ON BLOT TRANSFERS USING AVIDIN-BIOTIN TECHNOLOGY [J].
BAYER, EA ;
BENHUR, H ;
WILCHEK, M .
ANALYTICAL BIOCHEMISTRY, 1987, 161 (01) :123-131
[3]   ACCUMULATION OF BETA-CAROTENE IN HALOTOLERANT ALGAE - PURIFICATION AND CHARACTERIZATION OF BETA-CAROTENE-RICH GLOBULES FROM DUNALIELLA-BARDAWIL (CHLOROPHYCEAE) [J].
BENAMOTZ, A ;
KATZ, A ;
AVRON, M .
JOURNAL OF PHYCOLOGY, 1982, 18 (04) :529-537
[4]   ACCUMULATION OF METABOLITES BY HALOTOLERANT ALGAE AND ITS INDUSTRIAL POTENTIAL [J].
BENAMOTZ, A ;
AVRON, M .
ANNUAL REVIEW OF MICROBIOLOGY, 1983, 37 :95-119
[5]  
BENAMOTZ A, 1978, ENERGETICS STRUCTURE, P529
[6]  
BERMAN J, 1985, J BIOL CHEM, V260, P5240
[7]   SALT RELATIONS OF DUNALIELLA - FURTHER OBSERVATIONS ON GLYCEROL PRODUCTION AND ITS REGULATION [J].
BOROWITZKA, LJ ;
KESSLY, DS ;
BROWN, AD .
ARCHIVES OF MICROBIOLOGY, 1977, 113 (1-2) :131-138
[8]  
Borowitzka M.A., 1988, P27
[9]   CHARACTERIZATION OF A RICE GENE SHOWING ORGAN-SPECIFIC EXPRESSION IN RESPONSE TO SALT STRESS AND DROUGHT [J].
CLAES, B ;
DEKEYSER, R ;
VILLARROEL, R ;
VANDENBULCKE, M ;
BAUW, G ;
VANMONTAGU, M ;
CAPLAN, A .
PLANT CELL, 1990, 2 (01) :19-27
[10]   THE BIOCHEMISTRY OF P-GLYCOPROTEIN-MEDIATED MULTIDRUG RESISTANCE [J].
ENDICOTT, JA ;
LING, V .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :137-171