FLUORIDE PREVENTS DEGRANULATION OF THE AZUROPHILIC AND SPECIFIC GRANULES IN ELECTROPERMEABILIZED NEUTROPHILS

被引：6

作者：

NIESSEN, HWM

KESSELS, GCR

VERHOEVEN, AJ

机构：

[1] NETHERLANDS RED CROSS,DEPT BLOOD CELL CHEM,BLOOD TRANSFUS SERV,CENT LAB,1066 CX AMSTERDAM,NETHERLANDS

[2] UNIV AMSTERDAM,EXPTL & CLIN IMMUNOL LAB,AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1994年 / 55卷 / 04期

关键词：

NEUTROPHILS; DEGRANULATION; ELECTROPERMEABILIZATION; FLUORIDE;

D O I：

10.1002/jlb.55.4.489

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effect of A1F(4)- on the degranulation process in human neutrophils was investigated. In intact neutrophils, A1F(4)- induced degranulation, whereas in electropermeabilized neutrophils A1F(4)- did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca2+ and/or GTP-gamma-S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca2+ or by GTP-gamma-S but not degranulation induced by the combination of Ca2+ plus GTP-gamma-S. It is concluded that under suboptimal conditions of stimulation with Ca2+ or GTP-gamma-S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils.

引用

页码：489 / 495

页数：7

共 35 条

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