ENHANCEMENT OF HIV-1 PROTEINASE ACTIVITY BY HIV-1 REVERSE-TRANSCRIPTASE

被引：10

作者：

GOOBARLARSSON, L

LUUKKONEN, BGM

UNGE, T

SCHWARTZ, S

UTTER, G

STRANDBERG, B

OBERG, B

机构：

[1] UPPSALA UNIV,BMC,DEPT MOLEC BIOL,S-11111 UPPSALA,SWEDEN

[2] MEDIVIR AB,S-14144 HUDDINGE,SWEDEN

来源：

VIROLOGY | 1995年 / 206卷 / 01期

关键词：

D O I：

10.1016/S0042-6822(95)80054-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

HIV-1 reverse transcriptase (RT) was found to increase the activity of HIV-I proteinase in vitro and in eukaryotic cells. The effect of RT on proteinase activity was dose-dependent and independent of pH or salt concentration. The cleavage of sequences corresponding to all the naturally occurring cleavage sites that could be tested in vitro was enhanced. The effect of RT on cleavage was greatest at the cleavage site between RT and integrase. The enhancement of viral proteinase activity by the virus RT may contribute to regulation of the order and/or efficiency of cleavage at different sites during virus replication and maturation. (C) 1995 Academic Press, Inc.

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页码：387 / 394

页数：8

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