ISOLATION AND CHARACTERIZATION OF A COLLAGENOLYTIC ENZYME FROM BACILLUS-LICHENIFORMIS N22

被引：17

作者：

ASDORNNITHEE, S ^{[1
]}

AKIYAMA, K ^{[1
]}

SASAKI, T ^{[1
]}

TAKATA, R ^{[1
]}

机构：

[1] EHIME UNIV,COLL AGR,DEPT BIOTECHNOL,MATSUYAMA,EHIME 790,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1994年 / 78卷 / 04期

关键词：

D O I：

10.1016/0922-338X(94)90358-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A bacterium having collagenolytic activity was isolated from soil and identified as Bacillus licheniformis. The collagenolytic enzyme was obtained in a medium containing gelatin and purified to apparent homogeneity on the basis of SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 29,000, and hydrolyzed native, insoluble collagen. In addition, the enzyme hydrolyzed soluble collagen, gelatin, hemoglobin and casein, but not synthetic oligopeptides, usually used as substrates for collagenase assay.

引用

页码：283 / 287

页数：5

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