ELASTASE LEVELS IN SALVAGED BLOOD AND THE EFFECT OF CELL WASHING

被引：17

作者：

SIEUNARINE, K

LANGTON, S

LAWRENCEBROWN, MMD

GOODMAN, MA

PRENDERGAST, FJ

HELLINGS, M

机构：

[1] Department of Vascular Surgery, Perth, Western Australia

[2] Department of Royal Perth Hospital, Perth, Western Australia

[3] Department of Biochemistry, Fremantle Hospital, Fremantle, Western Australia

来源：

AUSTRALIAN AND NEW ZEALAND JOURNAL OF SURGERY | 1990年 / 60卷 / 08期

关键词：

autotransfusion; plasma elastase levels; white cell lysosomal granules;

D O I：

10.1111/j.1445-2197.1990.tb07441.x

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Blood was salvaged from the operating field of 16 orthopaedic and vascular operations and processed by the Shiley Dideco cell saver for retransfusion. Plasma elastase levels in the salvaged blood were used as a marker of white cell lysosomal granule release. The plasma level of this enzyme was measured by the technique described by Dreher et al. using the immuno‐activation (IMAC™) system (Merck‐Darmstadt, Federal Republic of Germany).1 The levels of the enzyme rose dramatically in the salvaged blood and were reduced to preoperative levels after washing in the cell saver. Washing of the salvaged blood before retransfusion is effective in reducing the plasma levels of white cell lysosomal contents which have the potential for producing harmful systemic effects. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：613 / 616

页数：4

共 32 条

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