N-DEGLYCOSYLATION AND IMMUNOLOGICAL IDENTIFICATION INDICATES THE EXISTENCE OF BETA-SUBUNIT ISOFORMS OF THE RAT GABA(A)-RECEPTOR

被引：36

作者：

BUCHSTALLER, A

ADAMIKER, D

FUCHS, K

SIEGHART, W

机构：

[1] UNIV VIENNA,PSYCHIAT KLIN,DEPT BIOCHEM PSYCHIAT,WAHRINGER GURTEL 18-20,A-1190 VIENNA,AUSTRIA

[2] RES INST LAB ANIM BREEDING,HIMBERG,AUSTRIA

来源：

FEBS LETTERS | 1991年 / 287卷 / 1-2期

关键词：

GABA(A)-RECEPTOR; N-DEGLYCOSYLATION; BETA-SUBUNIT; ANTIBODY;

D O I：

10.1016/0014-5793(91)80008-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-2- and beta-3-subunits of GABA(A) receptors purified from the brains of 5-10-day-old rats were investigated in the intact or completely N-deglycosylated state using the beta-subunit-specific monoclonal antibody bd-17 and polyclonal antibodies directed against synthetic amino acid sequences specific for the GABA(A) receptor beta-2- or beta-3-subunits. The present results seem to indicate the existence of two different isoforms of the beta-3-subunit and several different isoforms of the beta-2-subunit of the GABA(A) receptor which probably are produced by alternative splicing.

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页码：27 / 30

页数：4

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