DEVELOPMENT AND EVALUATION OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION, TYPING, AND SUBTYPING OF VESICULAR STOMATITIS-VIRUS

被引：11

作者：

ALONSO, A

MARTINS, MA

GOMES, MDD

ALLENDE, R

SONDAHL, MS

机构：

[1] Pan American Foot-and-Mouth Disease Center, Rio de Janeiro

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 1991年 / 3卷 / 04期

关键词：

D O I：

10.1177/104063879100300403

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An indirect sandwich enzyme-linked immunosorbent assay (ELISA) has been used for vesicular stomatitis virus (VSV) typing using sets of monovalent and polyvalent rabbit/guinea pig antisera for identification of VSV types New Jersey (VNJ) and Indiana (VIND). The VIND polyvalent antiserum (VIND-P) detects any strain of the 3 subtypes of the VIND type (VIND-1, VIND-2, and VIND-3) with the same strong reactivity. It is also possible to subtype the VIND strains using VIND-P rabbit antiserum as capture antibody and monovalent VIND-1, VIND-2, or VIND-3 guinea pig antisera as detector. The ELISA proposed has about 10 times more sensitivity and provides 10% more positive results than does the complement fixation 50% (CF50) test when epithelial samples are tested.

引用

页码：287 / 292

页数：6