THE DEVELOPMENT AND ASSESSMENT OF DNA AND OLIGONUCLEOTIDE PROBES FOR THE SPECIFIC DETECTION OF BACILLUS-ANTHRACIS

被引：21

作者：

HUTSON, RA

DUGGLEBY, CJ

LOWE, JR

MANCHEE, RJ

TURNBULL, PCB

机构：

[1] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,DIV BIOL,SALISBURY,WILTS,ENGLAND

[2] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,DIV BIOTECHNOL,SALISBURY,WILTS,ENGLAND

[3] CHEM & BIOL DEF ESTAB,DIV DEF MICROBIOL,SALISBURY,WILTS,ENGLAND

[4] USA,MED RES INST INFECT DIS,FREDERICK,MD

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1993年 / 75卷 / 05期

关键词：

D O I：

10.1111/j.1365-2672.1993.tb02803.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two DNA probes and a number of oligonucleotide probes were designed from the virulence factor genes of Bacillus anthracis. These probes were tested for specificity against 52 B. anthracis strains and 233 Bacillus strains encompassing 23 other species. A rapid slot blotting technique was used for screening the large numbers of isolates involved. All probes tested appeared to be specific for B. anthracis under high stringency conditions. These probes could differentiate between virulent and avirulent strains. The probes were also applied to the detection of B. anthracis in routine environmental and clinical samples. A non-radioactive hybridization and detection system based on digoxigenin-11-dUTP was developed.

引用

页码：463 / 472

页数：10

共 35 条

[1] DETECTION OF B19-PARVOVIRUS INFECTIONS BY A DOT BLOT HYBRIDIZATION ASSAY USING A DIGOXIGENIN-LABELED PROBE [J].