BIOCHEMICAL-CHARACTERIZATION OF A VASCULAR SMOOTH-MUSCLE CONTRACTING POLYPEPTIDE PURIFIED FROM PHONEUTRIA-NIGRIVENTER (ARMED SPIDER) VENOM

被引：20

作者：

MARANGONI, S

BORGES, NCC

MARANGONI, RA

ANTUNES, E

VIEIRA, CA

NOVELLO, JC

DOMONT, GB

GIGLIO, JR

OLIVEIRA, B

DENUCCI, G

机构：

[1] UNICAMP,DEPT PHARMACOL,POB 611,BR-13081 CAMPINAS,SP,BRAZIL

[2] UNICAMP,DEPT BIOCHEM,BR-13081 CAMPINAS,SP,BRAZIL

[3] UNIV SAO PAULO,RIBEIRAO PRETO MED SCH,DEPT BIOCHEM,BR-14049 RIBEIRAO PRE,SP,BRAZIL

[4] FED UNIV RIO DE JANEIRO,DEPT BIOCHEM,BR-21910 RIO JANEIRO,BRAZIL

来源：

TOXICON | 1993年 / 31卷 / 04期

关键词：

D O I：

10.1016/0041-0101(93)90173-G

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Crude Phoneutria nigriventer venom was fractionated by Sephadex, ion-exchange and reverse-phase high performance liquid chromatography. One protein (PNV1) with spasmogenic activity in rabbit vascular smooth muscle was isolated and biochemically characterized. PNV1 has 125 amino acid residues and a calculated mol. wt of 13,899. Special features of the amino acid composition of PNV1 are the presence of two disulfide bridges and the high percentage (27%) of Asx and Glx. The N-terminal amino acid sequence indicates that PNV1 is different from other polypeptides isolated from Phoneutria nigriventer venom.

引用

页码：377 / 384

页数：8

共 15 条

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