STABILIZATION OF CANDIDA LIPASE AGAINST ACETALDEHYDE BY ADSORPTION ONTO CELITE

被引：21

作者：

KAGA, H ^{[1
]}

SIEGMUND, B ^{[1
]}

NEUFELLNER, E ^{[1
]}

FABER, K ^{[1
]}

PALTAUF, F ^{[1
]}

机构：

[1] GRAZ TECH UNIV, INST BIOCHEM, SPEZIALFORSCH BEREICH BIOKATALYSE, A-8010 GRAZ, AUSTRIA

来源：

BIOTECHNOLOGY TECHNIQUES | 1994年 / 8卷 / 06期

关键词：

D O I：

10.1007/BF00154305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilization of Candida rugosa lipase (Amano AY-30) by adsorption onto Celite 545 led to a markedly improved performance of the enzyme: Firstly, the enzyme was almost completely resistant to deactivation by acetaldehyde, which is liberated as unavoidable by-product in acyl-transfer reactions with vinyl esters, and secondly, the enantioselectivity was enhanced up to three-fold.

引用

页码：369 / 374

页数：6

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