V-GENE AND C-GENE CONTRIBUTION IN CREATING ANTI-ALPHA-1,3 DEXTRAN ANTIBODIES IN MICE .2. CHARACTERISTICS OF IG-1B-TO-E PROTOTYPE RESPONSES

The light (L) and heavy (H) chain and the idiotypic (Id) composition of the antibody (Ab) plaque‐forming cells (PFC) and serum Ab specific for α‐1, 3 dextran have been characterized in murine strains exhibiting the CH‐lg‐l bto e allotypes and in their F1 hybrids with lg‐lal, BALB/c prototypes, The Ab response of the Ig‐lb to e mice to the α‐1, 3 dextran was low in the kappa (K) L chain class with only a minor, sporadic Ab in the lambda (λ) L chain class discernible after prolonged immunization. Two of a total of sixty‐eight C57BI/6 Ig‐lb mice, in a total of 318 Ig‐lb to e mice tested, exhibited, in late responses, a significantly elevated Ab in the λ L class at both serum and PFC levels, equalling at the PFC level the total non‐specific λ PFC values. An Id analysis showed this λ Ab and the K Ab to lack the Id relatedness to the three BALB/c α‐1, 3 dextran‐binding myeloma proteins (MP) Ab prototypes, J‐558, 104 E, and UPC‐102, exhibited by the λ Id’ Ab of the lg‐lal BALB/c prototypes and their F1 hybrids with the Ig‐lb to e prototypes. Furthermore, affinity differences could be detected by α‐1, 3 nigerodextrans, PFC inhibition analysis, between the late C57BI/6 anti‐α‐1, 3 dextran λ Id−Ab PFC and the λ Id+ Ab PFC of the BALB/c and their F1 progeny. Copyright © 1979, Wiley Blackwell. All rights reserved