DISAPPEARANCE OF CERTAIN ACIDIC ORGANELLES (ENDOSOMES AND LANGERHANS CELL GRANULES) ACCOMPANIES LOSS OF ANTIGEN PROCESSING CAPACITY UPON CULTURE OF EPIDERMAL LANGERHANS CELLS

被引:159
作者
STOSSEL, H [1 ]
KOCH, F [1 ]
KAMPGEN, E [1 ]
STOGER, P [1 ]
LENZ, A [1 ]
HEUFLER, C [1 ]
ROMANI, N [1 ]
SCHULER, G [1 ]
机构
[1] UNIV INNSBRUCK,DEPT DERMATOL,ANICHSTR 35,A-6020 INNSBRUCK,AUSTRIA
关键词
D O I
10.1084/jem.172.5.1471
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Freshly isolated epidermal Langerhans cells (LC) can actively process native protein antigens, but are weak in sensitizing helper T cells. During culture, when LC mature into potent immunostimulatory dendritic cells, T cell sensitizing capacity develops but antigen processing capacity is downregulated. Processing of exogenous antigens for class 11-restricted antigen presentation involves acidic organelles. We used the DAMP-technique to monitor acidic organelles at the ultrastructural level in fresh, as well as cultured, mouse and human LC. We observed that the loss of antigen processing capacity with culture ofLC was reflected by the disappearance ofcertain acidic organelles, namely endosomes (particularly early ones), and the hitherto enigmatic LC granules (“Birbeck Granules”). Our findings support the notion that endosomes are critical for antigen processing and suggest that LC granules might be involved as well. © 1990, Rockefeller University Press., All rights reserved.
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页码:1471 / 1482
页数:12
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