MONOCLONAL-ANTIBODIES TO QUEBEC STRAIN (Q17) OF BOVINE ROTAVIRUS

被引：6

作者：

CORNAGLIA, E ^{[1
]}

ELAZHARY, Y ^{[1
]}

ROY, RS ^{[1
]}

TALBOT, B ^{[1
]}

机构：

[1] UNIV SHERBROOKE, FAC SCI, DEPT BIOL, SHERBROOKE J1K 2R1, QUEBEC, CANADA

来源：

VETERINARY MICROBIOLOGY | 1990年 / 23卷 / 1-4期

关键词：

D O I：

10.1016/0378-1135(90)90159-S

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fourteen monoclonal antibodies (MAbs) against "Quebec" strain (Q17) of bovine rotavirus were isolated and characterized. Four were specific for viral protein Vp7 and ten were specific for viral protein Vp6. Five different isotypes were represented by this group of antibodies. All of the anti-Vp6 and none of the anti-Vp7 antibodies were sensitive to the effects of periodate on their antigen. The antibodies could be separated into three groups based on their relative resistance to the dissociation of their antigen-antibody complex by thiocynate. The MAbs cross-reacted of porcine the proteins of porcine and human rotaviruses both by immunoprecipitation and immunoblot analyses. These techniques revealed the differences in Mw of the viral proteins from different serotypes. © 1990.

引用

页码：283 / 294

页数：12

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