THE ESTABLISHMENT OF HUMAN T-CELL LINES REACTIVE WITH SPECIFIC PERIODONTAL BACTERIA

被引：14

作者：

ISHII, T ^{[1
]}

MAHANONDA, R ^{[1
]}

SEYMOUR, GJ ^{[1
]}

机构：

[1] UNIV QUEENSLAND,DEPT DENT,IMMUNOPATHOL LAB,PHYSIOL BLDG,BRISBANE,QLD 4072,AUSTRALIA

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1992年 / 7卷 / 04期

关键词：

T-CELL; PERIODONTOPATHIC BACTERIA;

D O I：

10.1111/j.1399-302X.1992.tb00029.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Human T cell lines (TCLs) were obtained by stimulation of peripheral blood mononuclear cells (PBMCs) with the 2 periodontopathic bacteria, Porphyromonas gingivalis FDC-381 and Fusobacterium nucleatum FDC-263. After the first round of stimulation and rest, the cells responded specifically to the bacteria originally used to establish each line. Throughout the culture period, the responsiveness of each of the TCLs to their specific bacteria increased. Phenotypic analysis of the TCLs revealed heterogeneity of cell types. In both TCLs approximately 80% of the cells were T cells, all of which bore the alpha-beta-T cell receptor. The P gingivalis-reactive TCL (PG-TCL) showed approximately equal proportions of CD4+ and CD8+ cells, whereas the F. nucleatum-reactive TCL (FN-TCL) was predominantly CD4+. The expression of CD25, HLA-DR, CD45RA and CD29 on these CD4+ cells varied throughout the culture period of 45 days. These results demonstrate that it is possible to establish long-term T cell lines reacting to specific periodontopathic bacteria.

引用

页码：225 / 229

页数：5

共 21 条

[1]

Akbar AN, Terry L, Timms A, Beverley PCL, Janossy G., Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells, J Immunol, 140, pp. 2171-2178, (1988)

[2]

Akbar AN, Timms A, Janossy G., Cellular events during memory T‐cell activation in vitro: the UCHL1 (180,000 MW) determinant is newly synthesized after mitosis, Immunology, 66, pp. 213-218, (1989)

[3]

Aspinall R, O'Gorman A., Increased yields of IL‐2 in media conditioned by MLA 144 cells, J Immunol Methods, 101, pp. 79-84, (1987)

[4]

Bird PS, Seymour GJ, Production of monoclonal antibodies that recognize specific and cross‐reactive antigens of Fusobacterium nucleatum, Infect Immun, 55, pp. 771-777, (1987)

[5]

Cole KL, Seymour GJ, Powell RN, Phenotypic and functional analysis of T‐cells extracted from chronically inflamed human periodontal tissues, J Periodontol, 58, pp. 569-573, (1987)

[6]

Hemler ME, Brenner MB, McLean JM, Strominger JL, Antigenic stimulation regulates the level of expression of interleukin 2 receptor on human T cells, Proc Natl Acad Sci USA, 81, pp. 2172-2175, (1984)

[7]

Ivanyi L, Lehner T., Stimulation of lymphocyte transformation by bacterial antigens in patients with periodontal disease, Arch Oral Biol, 15, pp. 1089-1096, (1970)

[8]

Ivanyi L, Lehner T., Lymphocyte transformation by sonicates of dental plaque in human periodontal disease, Archives of Oral Biology, 16, (1971)

[9]

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, Protein measurement with the Folin phenol reagent, J Biol Chem, 193, pp. 265-275, (1951)

[10]

Mahanonda R, Seymour GJ, Powell LW, Good MF, Halliday JW, Limit dilution analysis of peripheral blood T lymphocytes specific to periodontopathic bacteria, Clin Exp Immunol, 75, pp. 245-251, (1989)

← 1 2 3 →