COMPREHENSIVE ASSAY FOR PYRAZINAMIDE, RIFAMPICIN AND ISONIAZID WITH ITS HYDRAZINE METABOLITES IN HUMAN PLASMA BY COLUMN LIQUID-CHROMATOGRAPHY

被引：45

作者：

WALUBO, A

SMITH, P

FOLB, PI

机构：

[1] Department of Pharmacology, University of Cape Town, Medical School, Observatory

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 658卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0378-4347(94)00230-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A comprehensive assay for determination of pyrazinamide (PZA), rifampicin (RIF), isoniazid (INH) and hydrazine metabolites is described. The method involves organic solvent extraction of PZA and RIF, followed by derivatization of INH, monoacetylhydrazine (mHYD) and hydrazine (HYD) with salicylaldehyde and extraction with diethyl ether. Acetylisoniazid (acINH) and diacetylhydrazine (dHYD) were hydrolyzed to INH and mHYD, respectively, and processed as above. Using a gradient solvent programmer, PZA and RIF were analyzed on a C-8 (5 mu m) column at 248 nm, while INH and metabolites were analyzed on a C-18 (5 mu m) ODS2 column at 280 nm.

引用

页码：391 / 396

页数：6

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