SUBCELLULAR DISTRIBUTION AND POSSIBLE NATURE OF BINDING FOR C-14-DIBENAMINE AND C-14-PROPANOLOL IN CNS

The binding capacity of subcellular fractions, from the cat basal ganglia and brain stem, was studied using respectively α- and β-adrenergic blocking agents 14C-dibenamine and 14C-propanolol. In both cases the highest uptake was obtained in the nerve-ending membrane fractions and was interfered with norepinephrin. In the case of 14C-dibenamine the localization of the binding was in the junctional complex which includes the subsynaptic membrane. Both the 14C-dibenamine and 14C-propanolol in a bound form were extracted with organic solvents. Although the nature of the material binding these drugs could not be ascertained with certainty, several experiments suggest that it may be a special proteolipid present in the nerve-ending membranes. © 1969.