INTERCALATION OF ETHIDIUM-BROMIDE INTO A TRIPLE-STRANDED OLIGONUCLEOTIDE

被引：108

作者：

MERGNY, JL ^{[1
]}

COLLIER, D ^{[1
]}

ROUGEE, M ^{[1
]}

MONTENAYGARESTIER, T ^{[1
]}

HELENE, C ^{[1
]}

机构：

[1] MUSEUM NATL HIST NAT, BIOPHYS LAB,CNRS,UA 481,INSERM,U201,43 RUE CUVIER, F-75231 PARIS 05, FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 07期

关键词：

D O I：

10.1093/nar/19.7.1521

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresponding duplex. Energy transfer from base triplets to ethidium shows that ethidium is intercalated into the triple helix. The spectroscopic characteristics of ethidium intercalated into a triplex are similar to those observed for intercalation into duplex DNA.

引用

页码：1521 / 1526

页数：6

共 33 条

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