AFFINITY-CHROMATOGRAPHY OF OVINE CASEIN

被引：8

作者：

DALLOLIO, S

DAVOLI, R

RUSSO, V

机构：

[1] Istituto di Allevamenti Zootecnici, Università di Bologna, Reggio Emilia, 42100, Via Fratelli Rosselli

来源：

JOURNAL OF DAIRY SCIENCE | 1990年 / 73卷 / 07期

关键词：

affinity chromatography; ovine casein;

D O I：

10.3168/jds.S0022-0302(90)78847-9

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Sheep milk casein was separated into two fractions: one containing αs1-plus β- and the other αs2 plus κ-caseins by affinity chromatography on activated thiol-Sepharose 4B. Milk samples were from the Leccese breed with the most common electrophoretic pattern. Electrophoresis of the chromatographic fractions on SDS-PAGE and on starch urea gel at pH 8.6 and 1.7 clarified the electrophoretic pattern of whole casein. Acidic pH electrophoresis of the two fractions obtained by affinity chromatography may be useful for investigations on the polymorphism of the casein fractions. © 1990, American Dairy Science Association. All rights reserved.

引用

页码：1707 / 1711

页数：5

共 29 条

[1] ISOLATION PURIFICATION AND ANALYSIS OF 2 K-CASEIN-LIKE FRACTIONS FROM SHEEP CASEIN [J].