DEVELOPMENTAL AND TISSUE SPECIFIC CONTROL OF THE HEAT-SHOCK INDUCED 70-KDA RELATED PROTEINS IN THE FLESH FLY, SARCOPHAGA-CRASSIPALPIS

Heat shock (40-43°C) induces two proteins in Sarcophaga crassipalpis that are immunologically related to the heat shock 70 protein family in Drosophila. These Sarcophaga heat shock proteins, Mr of 65 and 72 kDa, show developmental and tissue-specific expression. Heat shock protein 65 is expressed in the brain and integument of third-instar larvae at 43°C. Expression of heat shock protein 65 in both tissues ceases at pupariation and heat shock protein 72 is the protein induced at 43°C throughout the rest of development. Tissue specificity for the expression of these two heat shock proteins can be observed in 3-day-old adult males: brain and integument express heat shock protein 72 while the terminalia and flight muscle express heat shock protein 65. Both a developmental and temperature switch can be seen in the male terminalia: day-1 terminalia express heat shock protein 72 at either 40 or 43°C, on day-2 heat shock protein 72 is expressed at 40°C but heat shock protein 65 is expressed at 43°C, thereafter the terminalia express primarily heat shock protein 65 at either heat shock temperature. Control of heat shock protein expression in S. crassipalpis is thus considerably more complex than the Drosophila literature suggests. © 1990.