WESTERN-BLOT-ANALYSIS OF CEREAL GRAIN PROLAMINS USING AN ANTIBODY TO CARBOXYL-LINKED INDOLEACETIC-ACID

被引：17

作者：

LEVERONE, LA ^{[1
]}

STROUP, TL ^{[1
]}

CARUSO, JL ^{[1
]}

机构：

[1] UNIV CINCINNATI,DEPT BIOL SCI,CINCINNATI,OH 45221

来源：

PLANT PHYSIOLOGY | 1991年 / 96卷 / 04期

关键词：

D O I：

10.1104/pp.96.4.1076

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A monoclonal antibody raised against carboxyl-linked IAA was used in Western blot analysis of storage proteins from kernels of Avena sativa, Pennisetum americanum, Sorghum bicolor, and Zea mays. IAA or an IAA-like molecule is associated with the ethanol-soluble protein fraction of the seed. Western blotting of commercial zein, the major storage protein of maize, along with physico-chemical evidence reported by Leverone et al. ([1991] Plant Physiol, 96: 1070-1075) indicated that IAA is linked with this prolamin. Results suggest that an IAA-prolamin association may be widespread throughout the Poaceae.

引用

页码：1076 / 1078

页数：3

共 23 条

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