OPTIMIZATION FOR A RECOMBINANT ESCHERICHIA-COLI FED-BATCH FERMENTATION

被引：19

作者：

CHEN, Q ^{[1
]}

BENTLEY, WE ^{[1
]}

WEIGAND, WA ^{[1
]}

机构：

[1] UNIV MARYLAND,AGR BIOTECHNOL CTR,COLLEGE PK,MD 20742

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 51-2卷

关键词：

OPTIMIZATION; RECOMBINANT ESCHERICHIA-COLI; FED-BATCH REACTOR; FSQP;

D O I：

10.1007/BF02933447

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The operating strategy that produces the maximum foreign protein expression for a fed-batch process is desired. This is achieved by using a feasible quadratic programming (FSQP) algorithm with a structured model that describes cell growth and product formation for recombinant E. coli. Optimization calculations for a fed-batch culture have not been performed with a model of this complexity up to this point. A constraint on the maximum cell concentration was included. For a fixed value of batch time, the results show that the optimal time profile of feed flow rate can increase the yield of foreign protein by 12-29% over a constant feed rate policy. Also, it was found that the computation time for the FSQP algorithm can be reduced significantly by considering suboptimal profiles of the feed rate, with a minor effect on calculated protein yield.

引用

页码：449 / 461

页数：13

共 12 条

[1] INVESTIGATION OF SUBPOPULATION HETEROGENEITY AND PLASMID STABILITY IN RECOMBINANT ESCHERICHIA-COLI VIA A SIMPLE SEGREGATED MODEL [J].