LACTATE BIOSENSOR BASED ON A LACTATE-DEHYDROGENASE NICOTINAMIDE ADENINE-DINUCLEOTIDE BIOCOMPOSITE

被引：33

作者：

WANG, J

CHEN, Q

机构：

[1] Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico

来源：

ELECTROANALYSIS | 1994年 / 6卷 / 10期

关键词：

LACTATE; BIOSENSOR; BIOCOMPOSITE;

D O I：

10.1002/elan.1140061007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new biocomposite. fabricated by incorporating lactate dehydrogenase (LDH) and nicotinamide adenine dinucleotide (NAD(+)) within a graphite-epoxy matrix, is used as a polishable (renewable) and robust biosensor for lactate. Highly sensitive and rapid amperometric biosensing is accomplished at relatively low potentials. The regeneration capability of the biocomposite probe circumvents problems of surface fouling inherent to the detection of NADH. Flow-injection detection of lactate, with a sample throughput of 60/h, detection limit of 5 x 10(-4) M, precision of 2.5% (RSD for n = 40), and linearity up to 2 x 10(-2) M, is illustrated.

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页码：850 / 854

页数：5

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