COMPARISON BETWEEN HIGH-RESOLUTION SELECTED-ION MONITORING, SELECTED REACTION MONITORING, AND 4-SECTOR TANDEM MASS-SPECTROMETRY IN QUANTITATIVE-ANALYSIS OF GIBBERELLINS IN MILLIGRAM AMOUNTS OF PLANT-TISSUE

被引：43

作者：

MORITZ, T

OLSEN, JE

机构：

[1] Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences

[2] Department of Plant Physiology and Microbiology, IBG, University of Tromsø, Tromsø

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 10期

关键词：

D O I：

10.1021/ac00106a010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Gibberellins (GAs) were quantified in semipurified plant extracts by combined gas chromatography/mass spectrometry(GC/MS). High-resolution selected ion monitoring (HR-SIM), selected reaction monitoring (SRM), and four sector tandem mass spectrometry (MS/MS) were compared. The best selectivity was found with four-sector MS/MS analysis, but the sensitivity was too low for analysis in milligram amounts of plant tissue. HR-SIM and SRM had similarly low limits of detection, except for the analyses of GA(29), but SRM provided better selectivity. The variations of sensitivity and selectivity for the different MS methods are discussed.

引用

收藏

页码：1711 / 1716

页数：6

相关论文

共 19 条

[1] THE IMMUNOASSAY OF GIBBERELLINS .2. QUANTITATION OF GA3, GA4 AND GA7 BY ULTRASENSITIVE SOLID-PHASE ENZYME IMMUNOASSAYS [J].

ATZORN, R ;

WEILER, EW .

PLANTA, 1983, 159 (01) :7-11

[2] DETERMINATION OF TETRACHLORODIBENZO-PARA-DIOXINS BY MASS-SPECTROMETRIC METASTABLE DECOMPOSITION MONITORING [J].

CHESS, EK ;

GROSS, ML .

ANALYTICAL CHEMISTRY, 1980, 52 (13) :2057-2061

[3] MEASUREMENT PARAMETER SELECTION FOR QUANTITATIVE ISOTOPE-DILUTION GAS CHROMATOGRAPHY-MASS SPECTROMETRY [J].

COLBY, BN ;

ROSECRANCE, AE ;

COLBY, ME .

ANALYTICAL CHEMISTRY, 1981, 53 (12) :1907-1911

[4] COMPARISON OF CALCULATION PROCEDURES FOR ISOTOPE-DILUTION DETERMINATIONS USING GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

COLBY, BN ;

MCCAMAN, MW .

BIOMEDICAL MASS SPECTROMETRY, 1979, 6 (06) :225-230

[5] QUANTITATIVE-ANALYSIS OF GIBBERELLINS BY ISOTOPE-DILUTION MASS-SPECTROMETRY - A COMPARISON OF THE USE OF CALIBRATION CURVES, AN ISOTOPE-DILUTION FIT PROGRAM AND ARITHMETICAL CORRECTION OF ISOTOPE RATIOS [J].

CROKER, SJ ;

GASKIN, P ;

HEDDEN, P ;

MACMILLAN, J ;

MACNEIL, KAG .

PHYTOCHEMICAL ANALYSIS, 1994, 5 (02) :74-80

[6]

CROZIER A, IN PRESS PHYSICOCHEM

[7]

Crozier Alan, 1992, Analytical Proceedings, V29, P422, DOI 10.1039/ap9922900422

[8] AUTOMATED GAS-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY ASSAY FOR TEBUFELONE AND A C-13, O-18-LABELED ANALOG IN PLASMA - APPLICABILITY TO ABSOLUTE BIOAVAILABILITY DETERMINATION [J].

DOBSON, RLM ;

KELM, GR ;

NEAL, DM .

BIOLOGICAL MASS SPECTROMETRY, 1994, 23 (02) :75-81

[9] SELECTED METASTABLE PEAK MONITORING - NEW, SPECIFIC TECHNIQUE IN QUANTITATIVE GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

GASKELL, SJ ;

MILLINGTON, DS .

BIOMEDICAL MASS SPECTROMETRY, 1978, 5 (09) :557-558

[10] PARAFILM - CONVENIENT SOURCE OF N-ALKANE STANDARDS FOR DETERMINATION OF GAS CHROMATOGRAPHIC RETENTION INDICES [J].

GASKIN, P ;

MACMILLA.J ;

FIRN, RD ;

PRYCE, RJ .

PHYTOCHEMISTRY, 1971, 10 (05) :1155-&