CA2+-INDEPENDENT NITRIC-OXIDE SYNTHASE ACTIVITY IN HUMAN LUNG AFTER CARDIOPULMONARY BYPASS

被引：19

作者：

DELGADO, R ^{[1
]}

ROJAS, A ^{[1
]}

GLARIA, LA ^{[1
]}

TORRES, M ^{[1
]}

DUARTE, F ^{[1
]}

SHILL, R ^{[1
]}

NAFEH, M ^{[1
]}

SANTIN, E ^{[1
]}

GONZALEZ, N ^{[1
]}

PALACIOS, M ^{[1
]}

机构：

[1] HERMANOS AMEIJEIRAS HOSP,CARDIOSURG UNIT,HAVANA,CUBA

来源：

THORAX | 1995年 / 50卷 / 04期

关键词：

CARDIOPULMONARY BYPASS; NITRIC OXIDE; NITRIC OXIDE SYNTHASE;

D O I：

10.1136/thx.50.4.403

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Background - Because surgery involving cardiopulmonary bypass induces a systemic inflammatory response, the effect of cardiopulmonary bypass on nitric oxide (NO) generation was investigated in human lung tissue. Methods - Nitric oxide synthase (NOS) activity was measured by the conversion of C-14-L-arginine to C-14-L-citrulline in tissue biopsy samples obtained before and after cardiopulmonary bypass. Results - The Ca2+-independent proof NO found before cardiopulmonary bypass was extremely low (1.5 (0.5) pmol citrulline/mg/min), but was increased after the bypass operation (23.6 (11) pmol/mg/min). Conclusions - Ca2+-independent NOS activity was detected in human lung after cardiopulmonary bypass. This finding may provide an important insight into the pathogenesis of the tissue damage and acute phase response observed after such surgery.

引用

页码：403 / 404

页数：2

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