TRANSGENIC RABBITS AS BIOREACTORS FOR THE PRODUCTION OF HUMAN GROWTH-HORMONE

被引：32

作者：

LIMONTA, JM ^{[1
]}

CASTRO, FO ^{[1
]}

MARTINEZ, R ^{[1
]}

PUENTES, P ^{[1
]}

RAMOS, B ^{[1
]}

AGUILAR, A ^{[1
]}

LLEONART, RL ^{[1
]}

DELAFUENTE, J ^{[1
]}

机构：

[1] CTR INGN GENET & BIOTECNOL,DIV MAMMALIAN CELL GENET,HAVANA 6,CUBA

来源：

JOURNAL OF BIOTECHNOLOGY | 1995年 / 40卷 / 01期

关键词：

BIOREACTOR; HGH; MILK; RABBIT; TRANSGENIC;

D O I：

10.1016/0168-1656(95)00026-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Gene farming is one of the most promising areas in modern biotechnology. To assay the potential usefulness of transgenic rabbits as bioreactors, one cell embryos were microinjected with a chimeric gene comprising 5' sequences from mouse whey acidic protein gene (mWAP) linked to the human growth hormone (hGH) gene. Transgenic animals were obtained and the presence of the foreign protein was detected in the milk and serum of these animals at levels of up to 50 mu g ml(-1) and 0.6 ng ml(-1), respectively. Founder transgenics were able to transmit the microinjected gene to the first filial generation in a Mendelian fashion. These results showed that transgenic rabbits could constitute a suitable system for the rapid production of recombinant proteins in the milk of lactating females.

引用

页码：49 / 58

页数：10

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