STEM-CELL FACTOR

Stem cell factor (SCF) is the ligand for the tyrosine kinase receptor c-Kit, which is expressed on both primitive and mature hematopoietic progenitor cells, In vitro, SCF synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-colony-stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF, In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone, Other potential clinical uses include ex vivo expansion protocols and in vitro culture for gene therapy.