QUANTITATIVE DETERMINATION OF MIXTURES OF ALKYL ETHERS OF D-GLUCOSE

Analysis of complex mixtures of methyl ethers of d-glucose by gas-liquid chromatography of their trimethylsilyl derivatives is described. The ratio of the specific retention volumes for α- and β-anomers varies with the total number of methyl substituents and their position on the d-glucose molecule. The ratio of the retention volume of any trimethylsilyl derivative relative to its methyl analogue is independent of the number of trimethylsilyl or methyl groups elsewhere in the molecule. Molar responses of the trimethylsilylated methyl ethers of d-glucose are almost independent of the number of methyl substituents. Quantitative analysis of mixtures of all d-glucose methyl ethers substituted at C-2, C-3, and/or C-6 is possible with two columns. © 1969.