SIMULTANEOUS MEASUREMENTS OF DNA AND PROTEIN-CONTENT OF MICROORGANISMS BY FLOW CYTOMETRY

被引：21

作者：

HUTTER, KJ ^{[1
]}

EIPEL, HE ^{[1
]}

机构：

[1] BIOPHYS SYST GMBH,D-6140 BENSHEIM,FED REP GER

来源：

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1979年 / 6卷 / 03期

关键词：

D O I：

10.1007/BF00508094

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The simultaneous measurement of protein and DNA content of yeast on a cell by cell basis is described. A problem associated with partially overlapping fluorescence emission bands of the two fluorochromes is discussed. The rapid flow cytometric assay will be useful to monitor cell growth in industrial fermentation processes. © 1979 Springer-Verlag.

引用

页码：223 / 231

页数：9

共 15 条

[1] CERCEX L, 1977, BRIT J CANCER, V46, P903
[2] RAPID, SIMULTANEOUS MEASUREMENT OF DNA, PROTEIN, AND CELL VOLUME IN SINGLE CELLS FROM LARGE MAMMALIAN-CELL POPULATIONS
CRISSMAN, HA
STEINKAMP, JA
[J]. JOURNAL OF CELL BIOLOGY, 1973, 59 (03) : 766 - 771
[3] CRISSMAN HA, 1976, PULSE CYTOPHOTOMETRY, P112
[4] DILLA MA, 1969, SCIENCE, V163, P1213
[5] IMPULSE FLUOROMETRY IN SINGLE CELLS IN SUSPENSIONS
DITTRICH, W
GOHDE, W
[J]. ZEITSCHRIFT FUR NATURFORSCHUNG PART B-CHEMIE BIOCHEMIE BIOPHYSIK BIOLOGIE UND VERWANDTEN GEBIETE, 1969, B 24 (03): : 360 - &
[6] GOHDE W, 1970, Z ANAL CHEM FRESENIU, V252, P328
[7] GOHDE W, 1976, PULSE CYTOPHOTOMETRY, P27
[8] Hutter K.-J., 1975, Chemie Mikrobiologie Technologie der Lebensmittel, V4, P29
[9] PROTEIN-CONTENT DISTRIBUTIONS IN POPULATIONS OF BAKERS-YEAST
HUTTER, KJ
EIPEL, HE
[J]. EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1978, 5 (03): : 203 - 206
[10] DIFFERENT STAGES OF DNA-SYNTHESIS DURING GROWTH OF SACCHAROMYCES-CEREVISIAE
HUTTER, KJ
GORTZ, T
EIPEL, HE
[J]. FEMS MICROBIOLOGY LETTERS, 1978, 3 (05) : 291 - 294

← 1 2 →