PHOSPHORAMIDATES .V. PROBABLE IDENTITY OF RAT LIVER MICROSOMAL GLUCOSE 6-PHOSPHATASE PHOSPHORAMIDASE AND PHOSPHORAMIDATE-HEXOSE PHOSPHOTRANSFERASE

被引：38

作者：

PARVIN, R

SMITH, RA

机构：

[1] Chemistry Department, Biochemistry Division, University of California at Los Angeles, Los Angeles

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 04期

关键词：

D O I：

10.1021/bi00832a058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The microsomal fraction from rat liver and from kidney exhibited phosphoramidase and phosphoramidate-hexose phosphotransferase activities. A comparison of these two enzyme activities with that of glucose 6-phosphatase revealed that all three activities behaved alike under a variety of conditions. Evidence suggesting the involvement of a single enzyme catalyzing these different activities was also obtained by additive experiments and by kinetic data showing mutual competitive inhibitions. Formation of phosphoryl-enzyme from 32P-labeled glucose 6-phosphate supports the suggestion of a phosphoryl-enzyme intermediate in the over-all reaction mechanism. © 1969, American Chemical Society. All rights reserved.

引用

页码：1748 / &

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