NUCLEOTIDE-METABOLISM BY MICROSOMAL UDP-GLUCURONYLTRANSFERASE AND NUCLEOSIDE DIPHOSPHATASE AS DETERMINED BY P-31 NMR-SPECTROSCOPY

被引：19

作者：

FINCH, SAE ^{[1
]}

SLATER, TF ^{[1
]}

STIER, A ^{[1
]}

机构：

[1] BRUNEL UNIV,DEPT BIOCHEM,UXBRIDGE UX8 3PH,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1979年 / 177卷 / 03期

关键词：

D O I：

10.1042/bj1770925

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

31P n.m.r. spectroscopy was used to study the nucleotide kinetics of UDP-glucuronyltransferase and associated reactions in the liver microsomal fraction. The effects of Mg2+ and EDTA on these reactions were investigated qualitatively. It was found that the rabbit microsomal fraction has no nucleoside pyrophosphatase activity, that UDP was immediately hydrolysed and that it was released from the microsomal surface. Reverse glucuronyltransferase could be demonstrated. The results are discussed with reference to functional coupling of UDP-glucuronyltransferase to other enzymes and the effects of Mg2+ and EDTA on the system.

引用

页码：925 / 930

页数：6

共 13 条

[1] SUMMARY OF A NOVEL, 3-COMPONENT REGULATORY MODEL FOR URIDINE-DIPHOSPHATE GLUCURONYLTRANSFERASE
BERRY, C
HALLINAN, T
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1976, 4 (04) : 650 - 652
[2] DWEK RA, 1977, NMR BIOL
[3] FOUTS JR, 1971, METHOD PHARMACOL, V1, P287
[4] KORNBERG A, 1951, PHOSPHORUS METABOL, V1, P392
[5] KURIYAMA Y, 1972, J BIOL CHEM, V247, P2979
[6] DIFFERENTIATION OF ENDOPLASMIC RETICULUM IN HEPATOCYTES .2. GLUCOSE-6-PHOSPHATASE IN ROUGH MICROSOMES
LESKES, A
SIEKEVIT.P
PALADE, GE
[J]. JOURNAL OF CELL BIOLOGY, 1971, 49 (02) : 288 - &
[7] HETEROGENEOUS DISTRIBUTION OF GLUCOSE 6-PHOSPHATASE IN RAT-LIVER MICROSOMAL FRACTIONS AS SHOWN BY ADAPTATION OF A CYTOCHEMICAL TECHNIQUE
LEWIS, JA
TATA, JR
[J]. BIOCHEMICAL JOURNAL, 1973, 134 (01) : 69 - +
[8] RAPID NAD+-LINKED ASSAY FOR MICROSOMAL URIDINE-DIPHOSPHATE GLUCURONYLTRANSFERASE OF RAT-LIVER AND SOME OBSERVATIONS ON SUBSTRATE-SPECIFICITY OF ENZYME
MULDER, GJ
VANDOORN, ABD
[J]. BIOCHEMICAL JOURNAL, 1975, 151 (01) : 131 - 140
[9] PERMEABILITY OF MICROSOMAL MEMBRANES ISOLATED FROM RAT-LIVER
NILSSON, R
PETERSON, E
DALLNER, G
[J]. JOURNAL OF CELL BIOLOGY, 1973, 56 (03) : 762 - 776
[10] EFFECT OF PHOSPHOLIPASE-C TREATMENT ON LATENCY AND SEDIMENTABILITY OF RAT-LIVER MICROSOMAL NUCLEOSIDE DIPHOSPHATASE
OTOOLE, K
[J]. FEBS LETTERS, 1975, 52 (02) : 180 - 184

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