SPECIES AND TISSUE-SPECIFICITY OF MAMMALIAN GTP CYCLOHYDROLASE-I MESSENGER-RNA

被引：34

作者：

GUTLICH, M

SCHOTT, K

WERNER, T

BACHER, A

ZIEGLER, I

机构：

[1] GSF MUNICH,UMWELT & GESUNDHEIT FORSCHUNGSZENTRUM,INST EXPTL HAMATOL,MUNICH,GERMANY

[2] GSF MUNICH,UMWELT & GESUNDHEIT FORSCHUNGSZENTRUM,INST SAUGETIERGENET,NEUHERBERG,GERMANY

[3] TECH UNIV MUNICH,LEHRSTUHL ORGAN CHEM & BIOCHEM,W-8046 GARCHING,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1171卷 / 02期

关键词：

TETRAHYDROBIOPTERIN; GTP CYCLOHYDROLASE-I; NORTHERN BLOT; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0167-4781(92)90112-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Northern blot analysis of rat RNA from cell lines and isolated organs with a specific rat cDNA probe detected two GTP cyclohydrolase I mRNA species of approx. 1.4 and 3.6 kb. The ratio between these two species varies between 0.6 and 2.4 in different rat organs. Using primers derived from highly conserved regions in the rat and Escherichia coli cDNA sequences a human GTP cyclohydrolase I probe was obtained by means of reverse transcription and PCR (polymerase chain reaction). The human PCR product consisting of 555 bp was cloned and sequenced. It shows a 92% identity with the published sequence of the rat gene. The analysis of various human cell lines with this specific probe shows only one species of GTP cyclohydrolase I mRNA with an approximate size of 3.6 kb.

引用

页码：133 / 140

页数：8

共 31 条

[1] THE MTRAB OPERON OF BACILLUS-SUBTILIS ENCODES GTP CYCLOHYDROLASE-I (MTRA), AN ENZYME INVOLVED IN FOLIC-ACID BIOSYNTHESIS, AND MTRB, A REGULATOR OF TRYPTOPHAN BIOSYNTHESIS [J].