INVOLVEMENT OF THE ZN-BINDING REGION OF TETANUS TOXIN IN B-RECOGNITION AND T-RECOGNITION - INFLUENCE OF ZN FIXATION

Tetanus toxin contains a metal-binding site for zinc, located in its light chain. The sequence accounting for Zn fixation is part of a predicted amphipathic helical secondary structure and corresponds to a putative T cell epitope according to Rothbard and Taylor (EMBO J. 7, 93-100, 1988). In this paper, we analyse the antigenic properties of two synthetic peptides (233-248 = P12 and 225-243 = P13) containing the Zn binding sequence. Our results show that peptide P13 contains a B and T epitope. The B epitope seems to be immuno-dominant whether the T epitope is at least DR2 restricted. Zn binding on P13 leads to a decrease in its recognition by both antibodies and T lymphocytes.