ELECTROPHORETIC TECHNIQUES FOR THE ANALYSIS OF SYNTHETIC AMYLOID BETA-A4-RELATED PEPTIDES

被引：12

作者：

SWEENEY, PJ

DARKER, JG

NEVILLE, WA

HUMPHRIES, J

CAMILLERI, P

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,ANALYT SCI,FRYTHE,WELWYN GARDEN CIT AL6 9AR,HERTS,ENGLAND

[2] SMITHKLINE BEECHAM PHARMACEUT,CHEM SUPPORT PEPTIDE GRP,HARLOW CM19 5AD,ESSEX,ENGLAND

[3] SMITHKLINE BEECHAM PHARMACEUT,ANALYT SCI,EPSOM KT18 5XQ,SURREY,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 212卷 / 01期

关键词：

D O I：

10.1006/abio.1993.1310

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two electrophoretic techniques have been developed for the analysis of synthetic β-A4 related peptides. The first is an acetic acid-urea-polyacrylamide gel electrophoresis denaturing system while the second employs capillary zone electrophoresis at pH 7. Both methods were calibrated to identify the state (monomeric or aggregate) of the longer amyloid fragments β-A4(1-40) and β-A4(1-43) during analysis. © 1994 Academic Press, Inc. All rights reserved.

引用

页码：179 / 184

页数：6

共 18 条

[1] SOLUTION STRUCTURES OF BETA PEPTIDE AND ITS CONSTITUENT FRAGMENTS - RELATION TO AMYLOID DEPOSITION [J].